Rapid determination of xanthine metabolites by high-performance liquid chromatography with electrochemical and UV detection
Altro
Data di Pubblicazione:
2013
Citazione:
Rapid determination of xanthine metabolites by high-performance liquid chromatography with electrochemical and UV detection / S. Benedetti, B. Brunetti, M.S. Cosio, E. Desimoni. ((Intervento presentato al 24. convegno Congresso della Divisione di Chimica Analitica della Società Chimica Italiana tenutosi a Sestri Levante nel 2013.
Abstract:
Purine bases and their derivatives play an important role in the functioning
of living systems. Purines are involved in many metabolic processes as
cofactors which play key roles in fundamental biological processes. In
particular, uric acid (UA), xanthine (XA) and hypoxanthine (HX) are
degradation products of purine metabolism in human beings and higher
primates.
The determination of xanthine can be interesting not only in biological
fluids, since involved in various diseases, but also in food industry for
quality control of the products. Several analytical methods such as
colorimetry, fluorometry, HPLC, mass spectrometry, anion exchange
chromatography, thin layer chromatography and capillary column gas
chromatography have been employed for measurement of purines [1, 2].
In the present study a simple and sensitive method using high-performance
liquid chromatography with electrochemical detection (HPLC-ED) at
several electrochemical sensors and with ultraviolet detection (HPLC-UV)
has been developed for the determination of purines in fish meat for the
evaluation of freshness.
The experimental parameters were systematically optimized for each analyte
of UA, XA and HX. For an efficient separation and detection of these
analytes, a Hypersil ODS column, a mobile phase consisting of 0.004 M
potassium phosphate buffer pH 5.8 with 1% (v/v) of methanol in isocratic
mode were used. This system couples the suitable separation power of
HPLC to an inherent sensitivity of electrochemical detection in a short run
time, and is capable of measuring purine metabolites with minimal sample
preparation and low injection volume.
The estimation of the fish freshness by monitoring the purines content was
also established with a low concentration level and very good
reproducibility.
[1] R. Devi, S. Yadav, C.S. Pundir, Analyst 137 (2012) 754-759.
[2] T.C. Burdett, C.A. Desjardins, R.Logan, N.R. McFarland, X. Chen,
M.A. Schwarzschild, Biomedical Chromatography 27 (2013) 122-129.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
S. Benedetti, B. Brunetti, M.S. Cosio, E. Desimoni
Link alla scheda completa: