Helicobacter pylori infection associates with a mucosal downregulation of ghrelin, negative regulator of Th1-cell responses
Articolo
Data di Pubblicazione:
2013
Citazione:
Helicobacter pylori infection associates with a mucosal downregulation of ghrelin, negative regulator of Th1-cell responses / O.A. Paoluzi, G. Del Vecchio Blanco, R. Caruso, I. Monteleone, F. Caprioli, M. Tesauro, M. Turriziani, G. Monteleone, F. Pallone. - In: HELICOBACTER. - ISSN 1083-4389. - 18:6(2013 Dec), pp. 406-412.
Abstract:
Background: Helicobacter pylori (Hp)-related gastritis is characterized by a predominant T helper (Th)1/Th17 cell immunity. Ghrelin (GR) has immunoregulatory properties and inhibits experimental Th cell-dependent pathology. Aims: To evaluate whether Hp infection associates with changes in GR expression and whether GR negatively regulates Th1/Th17 cytokines during Hp infection. Methods: GR expression was evaluated by real-time PCR in gastric biopsies taken from Hp-infected and Hp-uninfected patients and in gastric biopsies of Hp-negative subjects cultured with or without H. pylori culture supernatant. To examine whether GR regulates Hp-induced cytokine production, H. pylori-infected gastric biopsies were stimulated with GR, and interleukin (IL)-12, interferon (IFN)-γ and IL-4 transcripts were evaluated by real-time PCR. IL-12 and IFN-γ were also analyzed in lamina propria mononuclear cells (LPMCs) extracted from Hp-infected gastric biopsies and cultured with GR. Results: GR RNA transcripts were reduced in biopsies from Hp-infected patients. Treatment of Hp-negative gastric biopsies with Hp culture supernatant reduced GR RNA expression. GR dose-dependently inhibited RNA expression of IL-12 and IFN-γ but not IL-4 in ex vivo cultures of mucosal explants and in cultures of gastric LPMCs from Hp-positive patients. Conclusions: GR is downregulated in the gastric mucosa of H. pylori-infected patients. Such a defect could contribute to sustain the ongoing Th1-cell response.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
gastritis; ghrelin; helicobacter pylori; Th1 cells
Elenco autori:
O.A. Paoluzi, G. Del Vecchio Blanco, R. Caruso, I. Monteleone, F. Caprioli, M. Tesauro, M. Turriziani, G. Monteleone, F. Pallone
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