Phycocyanobilin positively modulates inflammatory genes in acute cerebral hypoperfusion in rats
Poster
Data di Pubblicazione:
2013
Citazione:
Phycocyanobilin positively modulates inflammatory genes in acute cerebral hypoperfusion in rats / J. Marín Prida, N. Pavón Fuentes, A. Llópiz Arzuaga, J.R. Fernández Massó, A. Cruz Ramírez, N. Polentarutti, F. Riva, E. Pentón Arias, A. Mantovani, G. Pentón Rol - In: Immunitas vis naturae: book of abstracts of the 15th International Congress of Immunology[s.l] : The Editors, 2013 Aug. - pp. 1036 (( Intervento presentato al 15. convegno International Congress of Immunology tenutosi a Milano nel 2013.
Abstract:
Stroke causes high mortality rates and serious disability worldwide.
Ischemic penumbra rescue is the main objective of acute
interventions, such as thrombolysis and neuroprotection, but they
show low translational success. Increasing evidence demonstrates
that inflammatory response is involved in the stroke cascade. Here we evaluated the effects of Phycocyanobilin (PCB), the C-
Phycocyanin linked tetrapyrrole, on gene expression in the anterior
cortex of hypoperfused brains of rats. The acute drop of cerebral
blood flow in this model is comparable to ischemic penumbra, thus
offering a reliable tool for studying molecular mechanisms. After the
permanent bilateral common carotid arteries occlusion, the animals
were treated therapeutically with saline or PCB. Samples were taken
24h after the surgery. Global gene expression was analyzed with
GeneChip Rat Gene ST 1.1 from Affymetrix, and the expression of
particular genes was assessed by the Fast SYBR Green RT-PCR
Master Mix method. The PCB treatment significantly modulated 190
genes (93 up- and 97 down-regulated) associated to several
immunological processes in both the regulatory and effector subsets.
Furthermore, PCB was able to positively modulate 13 genes mostly
related to a detrimental pro-inflammatory environment. Our results
support the view of major inflammatory mediators in acute cerebral
hypoperfusion, effectively counteracted by PCB. These results may
justify the application of PCB as a new acute disease modifying drug
against ischemic stroke.
Tipologia IRIS:
03 - Contributo in volume
Elenco autori:
J. Marín Prida, N. Pavón Fuentes, A. Llópiz Arzuaga, J.R. Fernández Massó, A. Cruz Ramírez, N. Polentarutti, F. Riva, E. Pentón Arias, A. Mantovani, G. Pentón Rol
Link alla scheda completa:
Titolo del libro:
Immunitas vis naturae: book of abstracts of the 15th International Congress of Immunology