Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders
Articolo
Data di Pubblicazione:
2013
Citazione:
Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders / A. Lange-Consiglio, A. Meucci, F. Cremonesi. - In: OPEN VETERINARY JOURNAL. - ISSN 2226-4485. - 3:1(2013), pp. 21-35.
Abstract:
The aim of this study was to assess the effect on boar spermatozoa quality of
in vitro storage in short and long-term
extenders by fluorescent multiple staining (FMS) and computer assisted semen analyzer (CASA).
Fresh ejaculates from three healthy, sexually mature boars were diluted with equal volumes of six short-term or three long-term commercial extenders
and stored at 19°C for 6 days (short-
term) or 12 days (long-term). The integrity of spermatozoa membranes was analyzed by FMS using propidium iodide,
5,5’,6,6’-tetrachloro-1,1’,3,3’ tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) and fluorescein isothiocyanate-conjugated peanut agglutinin(PNA). The results obtained from this staining were compared with spermatozoa motility assessed by CASA.
Our study showed that the number of viable spermatozoa with non-reacted acrosomes and intact mitochondria was
positively correlated with the rate of motile spermatozoa (r2>0.9)irrespective of the extender used. In all extenders
the number of motile spermatozoa
significantly decreased as preservation period increased (P<0.05).
FMS test is a potent indicator of sperm motility because it analyses mitochondrial integrity independently from observable alterations in motility. The best performing extenders were BTS for short-term storage and TRI-x-Cell for long-termstorage.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Boar ; CASA ; fluorescent staining ; motility ; semen storage
Elenco autori:
A. Lange-Consiglio, A. Meucci, F. Cremonesi
Link alla scheda completa: