Data di Pubblicazione:
2013
Citazione:
Preparation of Fluorescent Tubulin Binders / E. Riva, M. Mattarella, S. Borrelli, M. Christodoulou, D. Cartelli, M. Main, S. Faulkner, D. Sykes, G. Cappelletti, J.S. Snaith, D. Passarella. - In: CHEMPLUSCHEM. - ISSN 2192-6506. - 78:3(2013 Mar), pp. 222-226. [10.1002/cplu.201200260]
Abstract:
Thiocolchicine, taxol and cephalomannine have been used as building blocks for the preparation of four different fluorescent compounds designed to image the tubulin/microtubule network in cells. Thiocolchicine gave the best results and, in particular, the compound derived from conjugation with fluorescein minimally inhibits tubulin polymerization, is cell permeable and binds microtubules. Thus, it meets some of the demanding requirements for a new fluorescent dye that can secure a direct assay to evidence the tubulin/microtubules network in cells.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
chephalomannines ; fluorescence ; fluorescent binders ; osmium ; thiocolchicines ; tubulins
Elenco autori:
E. Riva, M. Mattarella, S. Borrelli, M. Christodoulou, D. Cartelli, M. Main, S. Faulkner, D. Sykes, G. Cappelletti, J.S. Snaith, D. Passarella
Link alla scheda completa: