Data di Pubblicazione:
2001
Citazione:
Oxidative stress signalling in the apoptosis of Jurkat T lymphocytes / R. Chiaramonte, E. Bartolini, P. Riso, E. Calzavara, D. Erba, G. Testolin , P. Comi, G.V. Sherbet. - In: JOURNAL OF CELLULAR BIOCHEMISTRY. - ISSN 0730-2312. - 82:3(2001), pp. 437-444.
Abstract:
The pathways of transduction of oxidative stress signals have been studied using the Jurkat T cell model
The oxidative stress was induced by exposure of the cells to 100 mM H2O2. DNA damage was detected within 15 min
after commencement of treatment. DNA damage repair occurred within about 1 h in cells exposed to oxidative stress for
15 min. In continuous exposure to stress, DNA repair was slower and control levels of DNA integrity were not reached.
DNA repair did not involve gene transcription. H2O2 at 100 mM caused cell death by necrosis as well as by apoptosis.
Both these processes were induced by 15 min exposure to the stress stimulus. However, some important differences
were found between necrosis and apoptosis. Necrosis was more rapid, began within an hour of treatment and continued
to increase during the full duration of the experiment. But apoptosis was seen after 4 h from treatment and was
conspicuous between 6 and 20 h after the start of treatment. The necrotic phase preceded apoptosis, although these did
show an overlap. In the necrotic phase, Bcl-2, Caspase 8 genes were down regulated. The 6±20 h phase characterised by
a marked increase in apoptosis is accompanied by the up regulation of both Bcl-2 and Caspase genes. Expression of the
Fas and p53 genes was not altered in either phase. We also analysed the levels of expression of the scavenging genes
whose gene products are involved in detoxi®cation. No modulation of the antioxidant enzymes, catalase, Cu/Zn superoxide
dismutase and glutatione peroxidase was detectable.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Antioxidant enzymes; Apoptosis; Apoptosis genes; DNA repair; Leukaemia cells; Oxidative stress signalling
Elenco autori:
R. Chiaramonte, E. Bartolini, P. Riso, E. Calzavara, D. Erba, G. Testolin , P. Comi, G.V. Sherbet
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