Fluorescence Resonance Energy Transfer Techniques to Study Ligand-Mediated Interactions of PPARs with Coregulators
Capitolo
Data di Pubblicazione:
2013
Citazione:
Fluorescence Resonance Energy Transfer Techniques to Study Ligand-Mediated Interactions of PPARs with Coregulators / N. Mitro, C. Godio, M. Crestani - In: Peroxisome Proliferator-Activated Receptors (PPARs) / [a cura di] M.Z. Badr, J. Youssef. - Clifton N.J. : Humana Press, 2013. - ISBN 978-1-62703-154-7. - pp. 219-227 [10.1007/978-1-62703-155-4_16]
Abstract:
The capacity to induce the association of peroxisome proliferator-activated receptors (PPARs) with different transcriptional coregulators is determined by the peculiar 3D-structure that the receptors adopt when bound with a specific ligand. The fluorescence resonance energy transfer assay is a technique widely used to evaluate coregulator recruitment to nuclear receptors induced by ligands. With this assay it is possible to quantitatively determine the interaction and the affinity of coregulators with PPARs when these receptors are complexed with ligands. Here, we describe the use of this technique to assess the preferential interaction and the affinity of PPARγ with coregulators as a function of the chemical structure of the bound ligand
Tipologia IRIS:
03 - Contributo in volume
Keywords:
Coactivators; Corepressors; Fluorescence resonance energy transfer; Nuclear receptors; Peroxisome proliferator-activated receptors; Protein-protein interaction
Elenco autori:
N. Mitro, C. Godio, M. Crestani
Link alla scheda completa:
Titolo del libro:
Peroxisome Proliferator-Activated Receptors (PPARs)