Data di Pubblicazione:
2012
Citazione:
Characterization of “in vivo” sampled VOCs of Bixa orellana and its seeds extracts properties / A. Giorgi, L. Brussolo, M. Mattara, L.M. Chiesa, S. Panseri. ((Intervento presentato al 13. convegno Congress of the International
Society for Ethnopharmacology tenutosi a Graz, Austria nel 2012.
Abstract:
The purpose of this study was to identify the volatile organic compounds (VOCs) profile of different organs of Bixa orellana plants
sampled “in vivo” in the North of Pará State, Brazil. Sampling was done during an expedition on February 2011 within the Itaputyr
project, an Italian-Brazilian experience of bottom-up cooperation for development. (1,2). Moreover, antioxidant capacity, phenolic
content and insect repellent activity of powdered seeds extracts (aqueous and ethanolic) were tested. Branches, leaves, fruits and
seeds were sampled "in vivo" using Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS) fibers with the HS-SPME
technique. Each plant’s part was enclosed in a customised teflon cage (manufactured by SNK, Inc, Fullerton, CA: 11 x 21 cm) into
which a manual SPME holder was inserted to extract the headspace. HS-SPME analyses were performed using a Trace GC Ultra
Gas Chromatograph coupled to a quadrupole Mass Spectrometer Trace DSQ (Thermo-Fisher Scientific; Waltham, MA, USA).
Volatile compounds detected in Bixa orellana seed headspace consisted mainly of sesquiterpenes, monoterpenes, and arenes. Most
monoterpenes and sesquiterpenes have been previously detected in other spices and plants (3). α-humulene is the major volatile
present in Bixa orellana seed extracts followed by D-germacrene, α-and β-pinene and γ-elemene. Some of the monoterpenes and
sesquiterpenes found in plant extracts have been previously described as having antimicrobial and repellent properties (4). Phenolic
content and antioxidat capacity, evaluated with Folin-Ciocalteur and DPPH tests respectively, were not so high, while preliminary
data on repellency against Aedes aegypti of three different dried seeds extracts (ethanol, hexane, ethanol /water) indicated a
significant activity
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
A. Giorgi, L. Brussolo, M. Mattara, L.M. Chiesa, S. Panseri
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