Knock-out of the plastid ribosomal protein L11 in Arabidopsis: effects on mRNA translation and photosynthesis
Articolo
Data di Pubblicazione:
2001
Citazione:
Knock-out of the plastid ribosomal protein L11 in
Arabidopsis: effects on mRNA translation and
photosynthesis / P. Pesaresi, C. Varotto, J. Meurer, P. Jahns, F. Salamini, D. Leister. - In: PLANT JOURNAL. - ISSN 0960-7412. - 27:3(2001 Dec 23), pp. 179-189. [10.1046/j.1365-313X.2001.01076.x]
Abstract:
The prpl11±1 mutant of Arabidopsis thaliana was identi®ed among a collection of T-DNA tagged lines on
the basis of a decrease in the effective quantum yield of photosystem II. The mutation responsible was
localized to Prpl11, a single-copy nuclear gene that encodes PRPL11, a component of the large subunit of
the plastid ribosome. The amino acid sequence of Arabidopsis PRPL11 is very similar to those of L11
proteins from spinach and prokaryotes. In the prpl11±1 mutant, photosensitivity and chlorophyll
¯uorescence parameters are signi®cantly altered owing to changes in the levels of thylakoid protein
complexes and stromal proteins. The abundance of most plastome transcripts examined, such as those
of genes coding for the photosystem II core complex and RbcL, is not decreased. Plastid ribosomal RNA
accumulates in wild-type amounts, and the assembly of plastid polysomes on the transcripts of the rbcL,
psbA and psbE genes remains mainly unchanged in mutant plants, indicating that lack of PRPL11 affects
neither the abundance of plastid ribosomes nor their assembly into polysomes. However, in vivo
translation assays demonstrate that the rate of translation of the large subunit of Rubisco (RbcL) is
signi®cantly reduced in prpl11±1 plastids. Our data suggest a major role for PRPL11 in plastid ribosome
activity per se, consistent with its location near the GTPase-binding centre of the chloroplast 50S
ribosomal subunit. Additional effects of the mutation, including the pale green colour of the leaves and a
drastic reduction in growth rate under greenhouse conditions, are compatible with reduced levels of
protein synthesis in plastids.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Arabidopsis thaliana, mutant, photosynthesis, plastid ribosome, Prpl11, T-DNA, translation
Elenco autori:
P. Pesaresi, C. Varotto, J. Meurer, P. Jahns, F. Salamini, D. Leister
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