Data di Pubblicazione:
2002
Citazione:
Gene-sequence-tag expression analyses of 1,800 genes
related to chloroplast functions / J. Kurth, C. Varotto, P. Pesaresi,
A. Biehl, E. Richly, F. Salamini,
D. Leister. - In: PLANTA. - ISSN 0032-0935. - 215:1(2002 Jan 31), pp. 101-109.
Abstract:
Quantification of the expression levels of nuclear
genes encoding plastid proteins under different
genetic or environmental conditions can contribute to
the genetic dissection of plastid functions. To facilitate
suchmeasur ements, a set of 1,827 Arabidopsis thaliana
genes coding for plastid proteins was PCR-amplified
from genomic DNA and spotted on nylon membranes to
generate an array of chloroplast-specific gene-sequencetags
(GSTs). The sensitivity and reliability of the experimental
system was evaluated and a procedure was
developed for detecting differential gene expression. The
GST array was found to serve as a reliable monitor of
changes in gene expression induced by environmental
and genetic alteration of chloroplast functions. Based on
comparisons of dark- versus light-grown seedlings, and
wild-type versus prpl11-1 plants, lists of differentially
expressed genes are provided which include 193/7 and
25/42 up/down-regulated genes, respectively. The cut-off
values for differential expression were 2.5-times (up) and
0.40 (down). Additional up-regulated genes withrelatively
low expression ratios (from 1.5- to 2.5-times) or
down-regulated withrelati vely highratio s (0.4–0.67) can
be accessed at the website: http://www.mpiz-koeln.
mpg.de/ richly/GST-array.html. A sample of genes
analysed by quantitative reverse transcription PCR
confirmed the expression profiles monitored by the GST
array. Differential hybridisation experiments with the
prpl11-1 mutant revealed the existence of regulatory
networks sensing the protein state of the chloroplast and
transmitting the signal to the nucleus
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
J. Kurth, C. Varotto, P. Pesaresi,
A. Biehl, E. Richly, F. Salamini,
D. Leister
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