The metal ion transporter IRT1 is necessary for iron homeostasis and ef®cient photosynthesis in Arabidopsis thaliana
Articolo
Data di Pubblicazione:
2002
Citazione:
The metal ion transporter IRT1 is necessary for iron
homeostasis and ef®cient photosynthesis in Arabidopsis
thaliana / C. Varotto, D. Maiwald, P. Pesaresi, P. Jahns, F. Salamini, D. Leister. - In: PLANT JOURNAL. - ISSN 0960-7412. - 31:5(2002 Aug 30), pp. 589-599. [10.1046/j.1365-313X.2002.01381.x]
Abstract:
The mutants irt1-1 and irt1-2 of Arabidopsis thaliana were identi®ed among a collection of T-DNA-tagged
lines on the basis of a decrease in the effective quantum yield of photosystem II. The mutations
responsible interfere with expression of IRT1, a nuclear gene that encodes the metal ion transporter
IRT1. In irt1 mutants, photosensitivity and chlorophyll ¯uorescence parameters, as well as abundance
and composition of the photosynthetic apparatus, are signi®cantly altered. Additional effects of the
mutation under greenhouse conditions, including chlorosis and a drastic reduction in growth rate and
fertility, are compatible with a de®ciency in iron transport. Propagation of irt1 plants on media
supplemented with additional quantities of iron salts restores almost all aspects of wild-type behaviour.
The irt2-1 mutant, which carries an En insertion in the highly homologous IRT2 gene of Arabidopsis
thaliana, was identi®ed by reverse genetics and shows no symptoms of iron de®ciency. This, together
with the ®nding that irt1-1 can be complemented by 35S::IRT1 but not by 35S::IRT2, demonstrates that,
although the products of the two genes are closely related, only AtIRT1 is required for iron homeostasis
under physiological conditions
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Arabidopsis thaliana; Iron transporter; IRT1; IRT2; Photosynthesis; T-DNA; Transposon
Elenco autori:
C. Varotto, D. Maiwald, P. Pesaresi, P. Jahns, F. Salamini, D. Leister
Link alla scheda completa: