Mutants for photosystem I subunit D of Arabidopsis thaliana: effects on photosynthesis, photosystem I stability and expression of nuclear genes for chloroplast functions
Articolo
Data di Pubblicazione:
2004
Citazione:
Mutants for photosystem I subunit D of Arabidopsis thaliana: effects on photosynthesis, photosystem I stability and expression of nuclear genes for chloroplast functions / A. Ihnatowicz, P. Pesaresi, C. Varotto, E. Richly, A. Schneider, P. Jahns, F. Salamini, D. Leister. - In: PLANT JOURNAL. - ISSN 0960-7412. - 37:6(2004 Jan 23), pp. 839-852. [10.1111/j.1365-313X.2004.02011.x]
Abstract:
In Arabidopsis thaliana, the D-subunit of photosystem I (PSI-D) is encoded by two functional genes, PsaD1 and PsaD2, which are highly homologous. Knock-out alleles for each of the loci have been identified by a combination of forward and reverse genetics. The double mutant psad1-1 psad2-1 is seedling-lethal, high-chlorophyll-fluorescent and deficient for all tested PSI subunits, indicating that PSI-D is essential for photosynthesis. In addition, psad1-1 psad2-1 plants show a defect in the accumulation of thylakoid multiprotein complexes other than PSI. Of the single-gene mutations, psad2 plants behave like wild-type (WT) plants, whereas psad1-1 markedly affects the accumulation of PsaD mRNA and protein, and photosynthetic electron flow. Additional effects of the psad1-1 mutation include a decrease in growth rate under greenhouse conditions and downregulation of the mRNA expression of most genes involved in the light phase of photosynthesis. In the same mutant, a marked decrease in the levels of PSI and PSII polypeptides is evident, as well as a light-green leaf coloration and increased photosensitivity. Increased dosage of PsaD2 in the psad1-1 background restores the WT phenotype, indicating that PSI-D1 and PSI-D2 have redundant functions
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Arabidopsis thaliana; Expression profile; Mutant; Photosynthesis; Photosystem I; PSI-D
Elenco autori:
A. Ihnatowicz, P. Pesaresi, C. Varotto, E. Richly, A. Schneider, P. Jahns, F. Salamini, D. Leister
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