Data di Pubblicazione:
2012
Citazione:
New insights in diagnoses of bovine subclinical and clinical mastitis / P. Roncada, C. Piras, H.A. Hussein, R. Turk, A. Soggiu, M. Kovacic, M. Samardzija, M. De Canio, A. Urbani, L. Bonizzi. ((Intervento presentato al convegno EuPA/BSPR proteomics meeting : new horizons and applications for proteomics tenutosi a Glasgow nel 2012.
Abstract:
Introduction: Research for biomarkers discovery of bovine mastitis aims to identify reliable biomarkers
for early detection and drug efficacy. Most of proteomic studies on mastitis have been performed on milk
and somatic cells. Although proteomic profile of bovine milk whey proteins have been well
characterized still poor information have been provided on serum and plasma proteomics of bovine
mastitis. Aim of this work is to extend the current knowledge on molecular circulating biomarkers of
mastitis including both sub-clinical and clinical animal group. Collected evidences showed
complementary data between oxidative stress response, lipid metabolism and the differential protein
expression.
Methods: The study has been performed using a classical biochemistry approach to evaluate
inflammatory process and through a comparative proteomic analysis for the detection of differentially
expressed proteins among subclinical and clinical groups.
Results: Inflammatory status was evaluated analyzing PON1 and PAF-AH hat were found to be down-regulated
during clinical mastitis. A shotgun-MS approach was used to evaluate the differences in high
abundant proteins. Moreover using 2D eletrophoresis were found ten differentially expressed proteins.
Among them, vitronectin-like protein and serpin A3-1 have been described because of their increased
Expression in subclinical mastitis group.
Conclusion: Vitronectin production is linked to the process of bacterial opsonization that involves the
complement cascade. lt indicates the presence of a strong bacterial infection. Serpin A3-1 is a protease
inhibitor and because of its overexpression during inflammation it has been well characterized.
Evaluation of profiling and concentration of these biomarkers during subclinical mastitis could be helpful
for the early diagnoses of this pathology.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
P. Roncada, C. Piras, H.A. Hussein, R. Turk, A. Soggiu, M. Kovacic, M. Samardzija, M. De Canio, A. Urbani, L. Bonizzi
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