Data di Pubblicazione:
2001
Citazione:
Pulmonary interstitial pressure and tissue matrix structure in acute hypoxia / G. Miserocchi, A. Passi, D. Negrini, M. Del Fabbro, G. De Luca. - In: AMERICAN JOURNAL OF PHYSIOLOGY. LUNG CELLULAR AND MOLECULAR PHYSIOLOGY. - ISSN 1040-0605. - 280:5(2001 May), pp. L881-L887.
Abstract:
Pulmonary interstitial pressure was measured via micropuncture in anesthetized rabbits in normoxia and after breathing 12% O(2). In normoxia [arterial PO(2) = 88 +/- 2 (SD) mmHg], pulmonary arterial pressure and pulmonary interstitial pressure were 16 +/- 8 and -9.6 +/- 2 cmH(2)O, respectively. After 6 h of hypoxia (arterial PO(2) = 39 +/- 16 mm Hg), the corresponding values were 30+/-8 and 3.5+/-2.5 cm H(2)O (P<0.05). Pulmonary interstitial proteoglycan extractability, evaluated by hexuronate assay after 0.4 M guanidinium hydrochloride extraction, was 12.3, 32.4, and 60.6 microg/g wet tissue in normoxia and after 3 and 6 h of hypoxia, respectively, indicating a weakening of the noncovalent bonds linking proteoglycans to other extracellular matrix components. Gel filtration chromatography showed an increased fragmentation of chondroitin sulfate- and heparan sulfate-proteoglycans during hypoxic exposure, accounting for a loss of extracellular matrix native architecture and basement membrane structure. Gelatin zymography demonstrated increased amounts of the proteolytically activated form of gelatinase B (matrix metalloproteinase-9) after hypoxic exposure, providing evidence that the activation of proteinases may play a role in hypoxia-induced lung injury.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Animals; Intercostal Muscles; Extracellular Space; Heparitin Sulfate; Blood Pressure; Chondroitin Sulfates; Rabbits; Organ Size; Molecular Weight; Anoxia; Pulmonary Artery; Chromatography, Gel; Hydrostatic Pressure; Lung; Extracellular Matrix; Hexuronic Acids; Matrix Metalloproteinase 9
Elenco autori:
G. Miserocchi, A. Passi, D. Negrini, M. Del Fabbro, G. De Luca
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