Data di Pubblicazione:
1996
Citazione:
Production of laccase by Botrytis cinerea and fermentation studies with strain F226 / M. Fortina, A. Acquati, P. Rossi, P. Manachini, C. DiGennaro. - In: JOURNAL OF INDUSTRIAL MICROBIOLOGY. - ISSN 0169-4146. - 17:2(1996), pp. 69-72. [10.1007/BF01570044]
Abstract:
After induction, seven strains ofBotrytis cinerea released into the culture broth considerable amounts of laccase in a brief production time. The set-up of a suitable production process was studied with a selected strain in a 10-L fermenter. The optimum fermentation conditions were a 3% inoculum with a high degree of sporulation, a simple medium containing 20 g L–1 of glucose and 2 g L–1 of yeast extract at pH 3.5, 2 g L–1 gallic acid as inducer, added after 2 days of growth, an agitation speed of 300 rpm, an aeration rate of 1.2 vvm and a temperature of 24°C. By optimizing the culture conditions, the enzyme activity reached 28 U ml–1 in 5 days with a specific activity of 560 U mg–1 protein. The best procedure to obtain a suitable crude enzyme preparation was concentration of the supernatant medium to 10% of the initial volume by ultrafiltration, followed by a fractional precipitation with ethanol. The optimum pH and temperature for laccase activity were 5.5 and 40°C, respectively, with syringaldazine as the substrate.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Botrytis cinerea; Laccase; Optimization; Scaling up
Elenco autori:
M. Fortina, A. Acquati, P. Rossi, P. Manachini, C. Digennaro
Link alla scheda completa: