PCR-mediated site-directed mutagenesis on PedB gene and HaeIII restriction as a rapid tool for discrimination among pediocin AcH/PA-1 producer strains
Articolo
Data di Pubblicazione:
2000
Citazione:
PCR-mediated site-directed mutagenesis on PedB gene and HaeIII restriction as a rapid tool for discrimination among pediocin AcH/PA-1 producer strains / D. Mora, M. Fortina, C. Parini, P. Manachini. - In: FOOD MICROBIOLOGY. - ISSN 0740-0020. - 17:4(2000), pp. 415-420.
Abstract:
Nucleotide sequences of amplified pedB genes from Pediococcus parvulus andLactobacillus plantarum pediocin AcH/PA-1 producer strains were performed. The obtained data were aligned with the published Pediococcus acidilactici pedB sequence, showing a single base substitution in the third codon position of a putative serine. A PCR-mediated site directed mutagenesis on pedB gene, followed by Hae III restriction analysis was then carried out with the aim to rapidly discriminate among pediocin AcH/PA-1 producer strains of different species.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Lactic-acid bacteria; cloning; cheese
Elenco autori:
D. Mora, M. Fortina, C. Parini, P. Manachini
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