Specific identification of Lactobacillus helveticus by PCR with pepC, pepN and htrA targeted primers
Articolo
Data di Pubblicazione:
2001
Citazione:
Specific identification of Lactobacillus helveticus by PCR with pepC, pepN and htrA targeted primers / M. Fortina, G. Ricci, D. Mora, C. Parini, P. Manachini. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - 198:1(2001), pp. 85-89.
Abstract:
Specific regions in three genes coding for aminopeptidases C and N, and a trypsin-like serine protease were selected as species-specific
primer sequences for rapid and reliable identification of Lactobacillus helveticus strains. The PCR procedures carried out gave specific 524-,
726- and 918-bp amplificates, with DNA isolated from L. helveticus. No PCR product was generated for closely related bacteria. The
amplification products were also screened for their species specificity in dot blot hybridization with representatives of the most closely
related genera and species and a number of other bacterial species.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Aminopeptidase and trypsin-like serine protease gene; Bacterial identification; Lactobacillus helveticus; Polymerase chain reaction; Species-specific probe
Elenco autori:
M. Fortina, G. Ricci, D. Mora, C. Parini, P. Manachini
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