Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products
Articolo
Data di Pubblicazione:
2005
Citazione:
Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products / P. Cremonesi, M. Luzzana, M. Brasca, S. Morandi, R. Lodi, C. Vimercati, D. Agnellini, G. Caramenti, P. Moroni, B. Castiglioni. - In: MOLECULAR AND CELLULAR PROBES. - ISSN 0890-8508. - 19:5(2005), pp. 299-305.
Abstract:
A multiplex PCR for the simultaneous detection of Staphylococcus aureus 23S rRNA, the coagulase and thermonuclease genes as well as the enterotoxin genes sea, sec, sed, seg, seh, sei, sej, sel was developed. The method was used to determine the presence of enterotoxigenic types for 93 S. aureus strains isolated from milk and dairy products. The data obtained by mPCR resulted comparable to those obtained by immunoassay methods. In addition, the mPCR assays also amplified some se genes, whose toxins are undetectable by immunoassay. Multiplex amplification can be obtained starting from 1 pg of DNA, showing the excellent specificity and high sensitivity of the assay. (copyright) 2005 Elsevier Ltd. All rights reserved.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Dairy products; Enterotoxigenic Staphylococcus aureus; Milk; Multiplex PCR
Elenco autori:
P. Cremonesi, M. Luzzana, M. Brasca, S. Morandi, R. Lodi, C. Vimercati, D. Agnellini, G. Caramenti, P. Moroni, B. Castiglioni
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