Effects of temperature and SDS on the structure of beta-glycosidase from the thermophilic archaeon Sulfolobus solfataricus
Articolo
Data di Pubblicazione:
1997
Citazione:
Effects of temperature and SDS on the structure of beta-glycosidase from the thermophilic archaeon Sulfolobus solfataricus / S. D’auria, R. Barone, M. Rossi, R.Nucci, G. Barone, D. Fessas, E. Bertoli, F. Tanfani. - In: BIOCHEMICAL JOURNAL. - ISSN 0264-6021. - 323:3(1997), pp. 833-840.
Abstract:
The effects of temperature and SDS on the three-dimensional organization and secondary structure of β-glycosidase from the thermophilic archaeon Sulfolobus solfataricus were investigated by CD, IR spectroscopy and differential scanning calorimetry. CD spectra in the near UV region showed that the detergent caused a remarkable change in the protein tertiary structure, and far-UV CD analysis revealed only a slight effect on secondary structure. Infrared spectroscopy showed that low concentrations of the detergent (up to 0.02%) induced slight changes in the enzyme secondary structure, whereas high concentrations caused the α-helix content to increase at high temperatures and prevented protein aggregation.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
The effects of temperature and SDS on the three-dimensional organization and secondary structure of beta-glycosidase from the thermophilic archaeon Sulfolobus solfataricus were investigated by CD, IR spectroscopy and differential scanning calorimetry. CD spectra in the near UV region showed that the detergent caused a remarkable change in the protein tertiary structure, and far-UV CB analysis revealed only a slight effect on secondary structure. Infrared spectroscopy showed that low concentrations of the detergent (up to 0.02 %) induced slight changes in the enzyme secondary structure, whereas high concentrations caused the alpha-helix content to increase at high temperatures and prevented protein aggregation.
Elenco autori:
S. D’auria, R. Barone, M. Rossi, R.Nucci, G. Barone, D. Fessas, E. Bertoli, F. Tanfani
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