Data di Pubblicazione:
1986
Citazione:
EFFECTS OF PANTETHINE ON CHOLESTEROL-SYNTHESIS FROM MEVALONATE IN ISOLATED RAT HEPATOCYTES / G. CIGHETTI, M. DELPUPPO, R. PARONI, G. GALLI, M.G. KIENLE. - In: ATHEROSCLEROSIS. - ISSN 0021-9150. - 60:1(1986 Apr), pp. 67-77. [10.1016/0021-9150(86)90089-4]
Abstract:
Results presented here show that when isolated rat hepatocytes are incubated with increasing concentrations
of [2-raC]mevalonolactone, incorporation of the substrate into cholesterol is progressively reduced.
Correspondingly, an increase of the incorporation of the substrate into precursors of cholesterol (methyl
sterols and squalene) occurs. These effects and the observed inhibition of HMGCoA reductase at high
mevalonolactone concentration (0.5 mM) are in agreement with those shown by others in cultured
hepatocytes.
Since pantethine was reported to affect cholesterol biosynthesis from mevalonate in cultured fibroblasts.
effects of its addition to hepatocyte incubations at low and high mevalonoiactone concentration were
studied. Neither the amount of radioactivity incorporated into cholesterol and in its sterol precursors nor
sterol levels were modified by pantethine when a mevalonolactone concentration (0.01 mM) that did not
alter the levels of intermediates of cholesterol synthesis was used. Pantethine was shown instead to
potentiate the decrease of mevalonate incorporation into cholesterol induced by high concentrations of
mevalonolactone (0.5 mM). Decrease of 3-hydroxy-3-methylglutaryl CoA reductase activity induced by 1
mM pantethine was twice that caused by mevalonolactone alone. These results may explain the fact that
both in laboratory animals and in humans pantethine administration is effective in reducins cholesterol
plasma levels in hyperlipidemic conditions.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cholesterol biosynthesis; Mevalonate; Pantethine; Rat hepatocytes
Elenco autori:
G. Cighetti, M. Delpuppo, R. Paroni, G. Galli, M.G. Kienle
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