PANTETHINE INHIBITS CHOLESTEROL AND FATTY-ACID SYNTHESES AND STIMULATES CARBON-DIOXIDE FORMATION IN ISOLATED RAT HEPATOCYTES
Articolo
Data di Pubblicazione:
1987
Citazione:
PANTETHINE INHIBITS CHOLESTEROL AND FATTY-ACID SYNTHESES AND STIMULATES CARBON-DIOXIDE FORMATION IN ISOLATED RAT HEPATOCYTES / G. CIGHETTI, M. DELPUPPO, R. PARONI, E. FIORICA, M.G. KIENLE. - In: JOURNAL OF LIPID RESEARCH. - ISSN 0022-2275. - 28:2(1987 Feb), pp. 152-161.
Abstract:
The effects of pantethine on cholesterol and fatty
acid metabolism were investigated in isolated rat hepatocytes.
Preincubation of the cells with pantethine induced a concentration-
dependent decrease of the radioactivity incorporated
into carbon dioxide and lipids in incubations with [2-14C]acetate.
When pantethine and the labeled substrate were simultaneously
added to the cell suspension, there was an enhancement of
carbon dioxide radioactivity at short incubation time (5 min)
whereas, at longer incubation time, values were comparable to
those of controls; lipid radioactivity, instead, was dramatically
reduced by pantethine even at short incubation time and
decreased further during the incubation, being 23% of that of
controls at 60 min. Analysis of the incubation medium showed
that pantethine induced a concentration- and time-dependent
release of acetate into the medium. Results of the effect of the
acetate concentration on the incorporation of [ 2-I4C]acetate
radioactivity into CO2 and lipids in control hepatocytes allowed
the conclusion that the above-described modifications induced
by pantethine are only partially attributable to the dilution of
the labeled substrate, and that catabolism of acetate to carbon dioxide is stimulated by the disulphide pantethine, whereas
cholesterol and fatty acid syntheses are inhibited
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
CHOLESTEROL SYNTHESYS; PATETHINE, PANTETHEINE; FATTY ACIDS; RAT HEPATOCYTES
Elenco autori:
G. CIGHETTI, M. DELPUPPO, R. PARONI, E. FIORICA, M.G. KIENLE
Link alla scheda completa: