PRE-COLUMN DERIVATIZATION OF AMINO-ACIDS WITH N,N-DIETHYL-2,4-DINITRO-5-FLUOROANILINE AND REVERSED-PHASE LIQUID-CHROMATOGRAPHIC SEPARATION
Articolo
Data di Pubblicazione:
1988
Citazione:
PRE-COLUMN DERIVATIZATION OF AMINO-ACIDS WITH N,N-DIETHYL-2,4-DINITRO-5-FLUOROANILINE AND REVERSED-PHASE LIQUID-CHROMATOGRAPHIC SEPARATION / I. FERMO, F.M. RUBINO, E. BOLZACCHINI, C. ARCELLONI, R. PARONI, P.A. BONINI. - In: JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL APPLICATIONS. - ISSN 0378-4347. - 433:C(1988 Dec 09), pp. 53-62. [10.1016/S0378-4347(00)80584-8]
Abstract:
A new method for the separation and quantification of primary and secondary amino acids by
reversed-phase high-performance liquid chromatography and spectrophotometric detection is described.
The use of the novel derivatizing reagent N,N-diethyl-2,4-dinitro-5-fluoroaniline yields derivatives
that are more stable to light and heat in solution than the traditional ones. A simple gradient
between acidic acetate buffer and acetonitrile allows the complete separation of 21 amino acids in 80
min at room temperature. The good reproducibility of peak areas and retention times allows the
application of this simple and low-priced method to the determination of amino acids in the range
50-500 pmol per residue.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
HPLC; AMINO ACIDS ANALYSIS; PLASMA; PRE-COLUMN DERIVATIZATION
Elenco autori:
I. Fermo, F.M. Rubino, E. Bolzacchini, C. Arcelloni, R. Paroni, P.A. Bonini
Link alla scheda completa: