Data di Pubblicazione:
1990
Citazione:
DETERMINATION OF CREATININE IN SERUM AND URINE BY A RAPID LIQUID-CHROMATOGRAPHIC METHOD / R. PARONI, C. ARCELLONI, I. FERMO, P.A. BONINI. - In: CLINICAL CHEMISTRY. - ISSN 0009-9147. - 36:6(1990 Jun), pp. 830-836.
Abstract:
We describe an HPLC ion-pair procedure for rapid and
specific evaluationof creatinineinserum and urine. We used
a 15 cm x 4.6 mmODS column with a 50/50 (by vol) mixture
of sodium decanesulfonic acid (10 mmol/L, pH 3.2) and
methanoland measuredabsorbance at 236 nm. Serum (100
tL) or 30-fold-diluted urine (100 L) was added to 400 pL of
acetone. After centrifugation,the supemates (300 L) were
dried, reconstituted with the mobile phase, and injected into
the HPLC. Assay precision was tested for concentrations of
10, 29, and 130mg/Land yielded, respectively, 3.1%, 2.1%,
and 1.1% for within-dayCV and 2.8%, 2.1%, and 2.2% for
totalCV. Analytical recovery was 102 (±6.7)%. Unearitywas
demonstrated in the 0-200 mg/L range for serum and 0-3.5
g/L range for urine (r 0.999). The detection limit for creatmine
(signal-to-noiseratio = 3) was 0.5 mg/L. We used
cimetidinefor internalstandardization.Correlationwasgood
between this procedureand the Jaff#{k2i3n3e}tic,the enzymatic
(creatinineamidohydrolase),and the Fuller’searth alkaline
picrate methods.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Chromatography; Reversed-phase cimetidine as internal standard
Elenco autori:
R. PARONI, C. ARCELLONI, I. FERMO, P.A. BONINI
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