Data di Pubblicazione:
2003
Citazione:
Endogenous proteolytic activity in a rat model of spontaneous cerebral stroke / L. Sironi, A.M. Calvio, S. Bellosta, B. Lodetti, U. Guerrini, M. Monetti, E. Tremoli, L. Mussoni. - In: BRAIN RESEARCH. - ISSN 0006-8993. - 974:1-2(2003 Jun 06), pp. 184-192.
Abstract:
We evaluated the expression of two extra-cellular protease systems in a model of spontaneous cerebrovascular pathology: spontaneously hypertensive stroke-prone rats (SHRSP). The appearance of brain damage in individual animals was imaged and followed by means of magnetic resonance imaging (MRI). In situ zymography of brain slices obtained 3 days after the appearance of brain damage showed an increase in plasminogen activator (PA)/plasmin activity that co-localised with the cerebral damage detected by MRI; there was also concomitant accumulation/activation of inflammatory cells in the damaged area. Proteolytic activity was inhibited by the urokinase-specific inhibitor amiloride but not by an antibody against tissue-type plasminogen activator (t-PA). SDS-PAGE zymography of brain extracts revealed the presence of 58 kDa plasminogen-dependent lysis areas in the ischemic and non-ischemic tissues, and a 33 kDa lysis area in ischemic tissue only. An antibody against t-PA inhibited the former, whereas the latter was inhibited by amiloride. The specific induction of urokinase-type plasminogen activator (u-PA) in the damaged tissue was further confirmed by the fact that both u-PA protein mass and mRNA were markedly increased in the damaged cerebral areas. Concomitant metalloproteinase-2 (MMP-2) activation was only observed in the damaged area. These data suggest that u-PA is expressed and selectively catalyses proteolysis in the injured area of spontaneous brain damage in SHRSP.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Rats ; Magnetic Resonance Imaging ; Animals ; Blotting, Western ; Rats, Inbred SHR ; Electrophoresis, Polyacrylamide Gel ; Matrix Metalloproteinase 2 ; Stroke ; Tissue Plasminogen Activator ; Urokinase-Type Plasminogen Activator ; Endopeptidases ; Reverse Transcriptase Polymerase Chain Reaction
Elenco autori:
L. Sironi, A.M. Calvio, S. Bellosta, B. Lodetti, U. Guerrini, M. Monetti, E. Tremoli, L. Mussoni
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