Determination of ceftazidime concentration in Mueller Hinton agar by high-performance liquid chromatography
Articolo
Data di Pubblicazione:
1996
Citazione:
Determination of ceftazidime concentration in Mueller Hinton agar by high-performance liquid chromatography / C. Arcelloni,M. Basile, R. Vaiani, P.A. Bonini, R. Paroni. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 742:1-2(1996 Aug 23), pp. 121-126.
Abstract:
A simple and rapid RP-HPLC method for the direct determination of ceftazidime (a beta-lactam antibiotic) in agar (Mueller Hinton Agar-II) was developed. The method, characterised by good precision (C.V.less than or equal to 7.9%) and linearity in the 5-200 mu g/ml range (r(2) greater than or equal to 0.998), showed high recovery from agar (104+/-8%) and a sensitivity limit of 1.4 mu g/ml. The analytical procedure allowed the determination of the ''true'' antibiotic concentration in the agar matrix. In addition, the small volume sample may allow a precise evaluation of the antibiotic levels point by point in the agar plates necessary to study the kinetics of diffusion of ceftazidime.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Antibiotics; Ceftazidime; Muellar Hinton agar
Elenco autori:
C. Arcelloni,M. Basile, R. Vaiani, P.A. Bonini, R. Paroni
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