The molecular structure of apolipoprotein A-II modulates the capacity of HDL to promote cell cholesterol efflux
Articolo
Data di Pubblicazione:
1996
Citazione:
The molecular structure of apolipoprotein A-II modulates the capacity of HDL to promote cell cholesterol efflux / F. Bernini, L. Calabresi, G. Bonfadini, G. Franceschini. - In: BIOCHIMICA ET BIOPHYSICA ACTA. L, LIPIDS AND LIPID METABOLISM. - ISSN 0005-2760. - 1299:1(1996), pp. 103-109.
Abstract:
The influence of apolipoprotein A-II (apoA-II) molecular structure on the capacity of high density lipoproteins (HDL) to promote cellular cholesterol efflux was investigated in cultured mouse peritoneal macrophages (MPM). Conversion by reduction and carboxamidomethylation of the naturally occurring dimeric apoA-II to its monomeric form in both native or reconstituted HDL did not change apolipoprotein secondary structure and lipoprotein size/composition. All particles containing monomeric apoA-II, i.e., native HDL, or reconstituted HDL with or without apoA-I, showed a higher ability to promote cholesterol efflux originating from plasma membrane and intracellular stores, compared to particles containing dimeric apoA-II. These findings indicate that apolipoprotein molecular structure is a major determinant of HDL capacity to promote cholesterol efflux from cells.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cholesterol efflux; HDL; Macrophage; Reconstituted HDL; Reverse cholesterol transport
Elenco autori:
F. Bernini, L. Calabresi, G. Bonfadini, G. Franceschini
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