Is the direct quantitation of antibiotics in agar by high-performance liquid chromatography useful?
Articolo
Data di Pubblicazione:
1998
Citazione:
Is the direct quantitation of antibiotics in agar by high-performance liquid chromatography useful? / C. Arcelloni, R. Vaiani, R. Paroni. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 812:1-2(1998 Jul 03), pp. 111-116.
Abstract:
The direct quantification of antibiotics in agar allows one to study the quality of the agar matrix, the kinetics of diffusion
and the bacteria–antibiotic interaction. Mueller–Hinton agar (MHA) plates from three manufacturers were tested using
HPLC and the disc diffusion test of ceftazidime (CAZ). Notable differences in the chromatographic profiles of MHA plate extracts from OXOID, DID and Becton Dickinson (BD) were shown, with a higher CAZ concentration after 24 h at 6 mm in BD P. aeruginosa inoculated plates (5.1+/-1.7 mg/ ml, n=6) vs. OXOID and DID (1.6+/-0.3 mg/ ml, n=12). BD plates gave also a different inhibition zone diameter (26+/-0.5 mm, n=3) with respect to DID and OXOID (29+/-0.5 mm, n=3).
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Agar plates; Antibiotics; Ceftazidime; Mueller-Hinton agar plates; Pharmaceutical analysis
Elenco autori:
C. Arcelloni, R. Vaiani, R. Paroni
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