Low-tech electrophoresis, small but beautiful, and effective: Electrophoretic titration curves of proteins
Articolo
Data di Pubblicazione:
1999
Citazione:
Low-tech electrophoresis, small but beautiful, and effective: Electrophoretic titration curves of proteins / E. Gianazza, I. Miller, I. Eberini, S. Castiglioni. - In: ELECTROPHORESIS. - ISSN 0173-0835. - 20:7(1999), pp. 1325-1338.
Abstract:
Migration across a stationary pH gradient results in the electrophoretic titration of a protein's dissociable groups. From the resulting curves, some properties of the protein may be derived, including overall amino acid composition and type of mutation between polymorphic variants, as well as range of stability or, for enzymes, of catalytic activity. Analysis with this technique is a stringent purity criterion; other applications allow the study of interacting systems and the planning of chromatographic fractionations based on differences in surface charge.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Review; Titration curves
Elenco autori:
E. Gianazza, I. Miller, I. Eberini, S. Castiglioni
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