Histological and immunohistochemical findings in thoracic lymph nodes of cattle with contagious bovine pleuropneumonia (CBPP).
Altro
Data di Pubblicazione:
1998
Citazione:
Histological and immunohistochemical findings in thoracic lymph nodes of cattle with contagious bovine pleuropneumonia (CBPP) / E. Scanziani, S. Paltrinieri, M. Boldini, G. Mandelli, V. Grieco, C. Monaci. ((Intervento presentato al convegno COST 826 “Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics” tenutosi a Thessaloniki nel 1998.
Abstract:
HISTOLOGICAL AND IMMUNOHISTOCHEMICAL FINDINGS IN THORACIC LYMPH NODES OF CATTLE WITH CONTAGIOUS BOVINE PLEUROPNEUMONIA (CBPP)
E. Scanziani, S. Paltrinieri*, M. Boldini**, G. Mandelli, V. Grieco, C. Monaci**
Istituto di Anatomia Patologica Veterinaria e Patologia Aviare - Milano; * Istituto di Patologia Generale Veterinaria - Milano; ** Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia - Brescia
Introduction
Since the 1550 the contagious bovine pleuropneumonia (CBPP) spred all of the world. The prophilaxis and the increasing knowledges of the aetiology and the pathogenesis of the disease led to a decrease of the prevalence of the CBPP with a complete eradication from many countries: it was eradicated from the USA in 1982 and from Australia in 1967. In this century the CBPP was confined in some endemic african countries. In Europe some outbreaks of this disease occured in 1958 in Portugal (Ferronha et al. 1990) and between 1980 and 1984 in Spain, Portugal and France. At the end of 1990 the CBPP has been diagnosed in Lombardy, Northern Italy (Guadagnini et al., 1991). The clinico-pathological findings of CBPP are not specific: similar patterns of fibrinous pleuropneumonia are detectable in diseases such as the IBR, non specific mycoplasmosis etc... (Mandelli, 1989). The isolation and identification of Mycoplasma mycoides subsp. mycoides from infected animals is the only one reliable diagnostic test, but have many methodological problems (Al Aubaidi and Fabricant, 1970 Cottral 1978). Serological diagnosis is hampered either by false positive cattles due to cross-reactivity between Mycoplasma mycoides subsp. mycoides and other mycoplasmas or false negative animals in early and late phases of the disease.
Recently Mycoplasma mycoides subsp. mycoides antigen has been detected in the tissues by immunofluorescence (Ferronha et al., 1988) and immunohistochemistry (Ferronha et al. 1990; Scanziani et al. 1991a; Scanziani et al., 1991b): the localisation and the pattern of the positivity was variable with the evolutive step of the disease: in the acute phase antigens were detectable in alveolar macrophages as well as in the interlobular septa where a diffuse and weak positivity was found; with chronicity a strong positivity was found in the macrophages of fibrotic septa and in the follicular center of the broncho-alveolar associated lymphoid tissue (BALT). Necrotic debris in sequestra as well as the macrophages on their walls were strongly positive (Scanziani et al., 1991).
The lymph nodes only in the acute cases show gross pathological lesions: lymphoid hyperplasia and ectasia of the sinuses with a large number of macrophages were histologically detectable in the acute cases, while follicular hyperplasia with a large germinal center and fibrosis were present with chronicity. Large necrosis are rarely detectable: most frequently histology showed necrotic debris in the sinuses. In preliminary studies we detected Mycoplasma mycoides subsp. mycoides antigens in the lymph nodes (Scanziani et al. 1991a; Scanziani et al., 1991b)
The present work was assesed to study histological lesions and to detect the presence and localization of Mycoplasma mycoides subsp. mycoides antigens in thoracic lymph nodes of cattle affected by CBPP and to compare the results with bacteriological and serological positivity.
Matherial and methods
Animals
For the present study XX adult Holstein-Freisian cattle at slaughtering were selected. They came from 5 dairy herds on wich CBPP were present. All the animals were affected by CBPP: the diagnosis was based on the presence of tipical lung lesions and on the isolation of M.mycoides from the lungs.
For each animal macroscopic lesions of the lungs and thoracic lymph nodes were recor
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
E. Scanziani, S. Paltrinieri, M. Boldini, G. Mandelli, V. Grieco, C. Monaci
Link alla scheda completa: