Immunotoxin mediated killing of clonable T-lymphocytes infiltrating an irreversibly rejected human renal allograft
Articolo
Data di Pubblicazione:
1989
Citazione:
Immunotoxin mediated killing of clonable T-lymphocytes infiltrating an irreversibly rejected human renal allograft / S. Siena, M. Bregni, A. Formosa, B. Brando, D. A. Lappi, G. Bonadonna, A. M. Gianni. - In: JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS. - ISSN 0393-974X. - 3:2(1989), pp. 84-88.
Abstract:
We isolated and treated in vitro with a novel CD5-specific saporin immunotoxin, referred to as OKT1-SAP, the cells infiltrating an irreversibly rejected renal allograft from a patient who rejected while on cyclosporine plus steroids and then failed to respond to multiple courses of high-dose steroids, intravenous OKT3 antibody, and local irradiation to the graft. We report here that under experimental conditions achievable in vivo the immunotoxin OKT1-SAP was capable of eliminating in vitro more than 95% of clonable T-lymphocytes infiltrating the rejected allograft of this patient despite their resistance to previous aggressive immunosuppression. To our knowledge, this is the first report of an immunotoxin-mediated suppression of the clonogenic growth of rejected renal allograft infiltrating T-lymphocytes.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Antigens, Differentiation ; Kidney Transplantation ; Humans; Cell Separation; Plant Proteins ; Immunotoxins ; Phenotype ; Antibodies, Monoclonal ; Graft Rejection ; Cells, Cultured ; Adult ; Antigens, CD5 ; N-Glycosyl Hydrolases ; Ribosome Inactivating Proteins, Type 1 ; Flow Cytometry; Fluorescent Antibody Technique ; Male ; T-Lymphocytes ; Immunosuppression
Elenco autori:
S. Siena, M. Bregni, A. Formosa, B. Brando, D. A. Lappi, G. Bonadonna, A. M. Gianni
Link alla scheda completa: