Data di Pubblicazione:
2009
Citazione:
Analysis of canine haematopoietic neoplasms by using Sysmex the XT-2000iV / S. Paltrinieri, M.E. Gelain, G. Rossi, S. Comazzi. ((Intervento presentato al convegno Sysmex European Haematology Symposium 2009 tenutosi a Istanbul nel 2009.
Abstract:
Background and objectives
In dogs the differentiation between reactive and neoplastic proliferation of blood cells could be difficult and slide review by experienced hemathologist or expensive techniques such as flow cytometry are often required. The possibility to quickly identify neoplastic cells in blood and to predict the leukemic lineage could be useful to select the patients that need ancillary diagnostic test. In human medicine, automated methods to detect neoplastic cells and minimal residual disease in acute leukaemia have been developed. Also in veterinary medicine, the ability to identify leukaemic cells could be enhanced by using automated analyser such as Sysmex XT-2000iV (Sysmex, Kobe, Japan), which can analyze blood samples from several animal species. In addition, the analyser allows the operator to gate cell populations and to save these gates to analyse future samples or to re-gate specimens already stored in the database. This instrument would thus be used to preliminarily approach samples from dogs with hematopoietic neoplasms. In a previous study, we defined the gating procedures to design disease-specific gates able to differentiate chronic leukemia, acute leukemia, subleukemic conditions and reactive leukocytosis in dogs. The aim of this study is to collect information about the possible application of this instrumental approach in routine practice. Specifically, we would assess whether: 1) The analysis of data generated by Sysmex XT2000iV from eukemic vs non leukemic cases can differentiate “reactive” vs neoplastic conditions; 2) the analysis of data collected during the follow up can be used as a prognostic factor in luekemic and non leukemic cases.
Material and methods
Samples and diagnostic approach
This study was done on 163 canine blood samples classified in the following groups based on haematology, bone marrow and lmph node cytology and immunophenotyping:
- A: leukaemia (N=46), further sub-classified as acute myeloid leukemia (AML, N=4), acute lymphoid leukemia (ALL, N=12), stage V lymphoma (N=14), chronic lymphocytic leukemia (CLL, N=15), systemic mastocytosis (N=1)
- Group B: neoplasia without blood involvement (N=41): lymphoma (N=35), mast cell tumour (N=5), hystiocytic sarcoma (N=1) on which cytology and immunophenotyping did not reveal the presence of neoplastic cells in peripheral blood.
- Group C: non neoplastic reactive condition (N=33), with increased total WBC number - Group D: Healthy dogs (N=43): dogs without clinical or haematological changes.
Analysis of Sysmex data and scattergrams
the following parameters provided by the instrument were recorded and analyzed:
- Total WBC counts in the WBC-BASO channel.
- WBC flags such as the “positive” flag and/or the “grey area” WBC-DIFF differential.
- High fluorescence intensity (HFI) events: events included in an “HFI gate” extending from the top of the normal canine profile (SFL level: 150) to the top of the scattergram.
- Lysis resistant region (LRR) events: events included within a “LRR gate”, extending from above the few events classified as “basophils” to the upper part of the scattergram.
- Scattergram profiles: The shape of the scattergrams was visually assessed by two independent observers, which classified each case, as normal (N), acute leukaemia/V stage lymphoma (AL), chronic lymphocytic leukaemia (CL) or reactive leukocytosis (RL).
Data anlysis and interpretation
Four different approaches were followed:
Approach 1: diagnostic performance of each single parameter (WBC count, HFI or LRR events, presence of WBC flags and shape of the scattergram)
Approach 2: definition of a diagnostic algorithm including all the parameters mentioned above. The cut-off values employed for HFI, LRR a
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
S. Paltrinieri, M.E. Gelain, G. Rossi, S. Comazzi
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