Data di Pubblicazione:
2007
Citazione:
ISOLATION AND PURIFICATION OF LIGNOPEROXIDASE
FROM THE MUSHROOM Pleurotus ostreatus / S.Y. Mirzaakhmedov, Z.F. Ziyavitdinov, Z.R. Akhmedova, A.B. Saliev, D.T. Ruzmetova, S.T. Azizov, D. Fessas, S. Iametti. - In: CHEMISTRY OF NATURAL COMPOUNDS. - ISSN 0009-3130. - 43:6(2007), pp. 682-684. [10.1007/s10600-007-0227-6]
Abstract:
The accumulation dynamics of lignolytic enzymes in culture media of the basidiomycetes Panus tigrinus,
Pleurotus ostreatus, Fomes fomentarus, and the micromycete Aspergillus terreus were studied during the
incubation period. It was found that Pleurotus ostreatus is the most active producer of lignoperoxidase
enzymes among the studied fungi. Gel filtration and ion-exchange chromatography were used to isolate a
homogeneous enzyme with lignoperoxidase activity. The maximum activity was found at pH 2.7 and 29°C.
Gel electrophoresis determined the molecular weight (44 kDa).
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Chromatography; Electrophoresis; Ligninase; Lignoperoxidase activity; Pleurotus ostreatus
Elenco autori:
S.Y. Mirzaakhmedov, Z.F. Ziyavitdinov, Z.R. Akhmedova, A.B. Saliev, D.T. Ruzmetova, S.T. Azizov, D. Fessas, S. Iametti
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