Size-sieved subpopulations of mesenchymal stem cells from intervascular and perivascular equine umbilical cord matrix
Articolo
Data di Pubblicazione:
2011
Citazione:
Size-sieved subpopulations of mesenchymal stem cells from intervascular and
perivascular equine umbilical cord matrix / B. Corradetti, A. Lange-Consiglio, M. Barucca, F. Cremonesi, D. Bizzaro. - In: CELL PROLIFERATION. - ISSN 0960-7722. - 44:4(2011 Aug), pp. 330-342.
Abstract:
Objectives: Umbilical cord matrix (UCM) has been recently proposed as an alternative source of mesenchymal stem cells (MSCs). The aim of this study was to isolate and characterize presumptive stem cells
from intervascular and perivascular equine UCM and to obtain homogeneous subpopulations from both sites.
Materials and methods: Umbilical cords were processed for retrieval of MSCs. Unsieved cells from intervascular and perivascular portions were evaluated for cell cycle analysis and for immunophenotyping
by flow cytometry. Cells from each site were
separated into larger and smaller sieved populations using multi-dishes with 8-lm pore transwell inserts.
Each cell population was characterized in terms of renewal capability, specific marker expression and differentiation potential. Cryopreservation was performed on sieved cells only.
Results: Cells from both areas expressed MSC and pluripotential specific markers and were able to differentiate into mesodermic and ectodermic lineages.
The sieving procedure yielded two relatively homogeneous subpopulations with comparable characteristics.
Surprisingly, after sieving, large intervascular and small perivascular cells were the most rapidly replicating cells [20.53 and 19.49 cell population
doublings (PD) after 31 days respectively] and also showed higher fibroblast colony forming unit frequency. Unsieved cell populations were used as controls, and showed PD of 9.42(intervascular cells)
and 8.54 (perivascular cells) after 31 days.
Conclusions: Here, cells from UCM represented an intermediate stage between pluripotent embryonic and adult stem cells. Size-sieving can be used to isolate more rapidly proliferating cell populations.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Umbilical cord matrix ; mesenchymal stem cells ; size-sieving ; equine
Elenco autori:
B. Corradetti, A. Lange-Consiglio, M. Barucca, F. Cremonesi, D. Bizzaro
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