Data di Pubblicazione:
2011
Citazione:
POMEGRANATE ELLAGITANNINS ANTAGONIZE IN VITRO THE INFLAMMATORY RESPONSE INVOLVED IN MALARIA / E. Sangiovanni, M. Dell'Agli, G.V. Galli, M. Bulgari, N. Basilico, S. Romeo, D. Bhattacharya, D. Taramelli, E. Bosisio. ((Intervento presentato al convegno Italian Malaria Network tenutosi a Roma nel 2011.
Abstract:
Malaria is the infectious disease that causes
more deaths worldwide, approximately 2
million deaths each year and about 250
million infected individuals. The eastern
province of Orissa (India) is an area endemic
for both Plasmodium falciparum (Pf) and
Plasmodium vivax. Malaria patients receive a herbal preparation named OMARIA (Orissa Malaria Research
Indigenous Attempt), made of sun-dried rind of the immature P. granatum fruits (Pg). The effect could be attributed to different constituents of a fraction enriched in tannins (Pg-FET), namely ellagic acid (EA), and punicalagin (Punic), which inhibited in vitro
the growth of Pf asexual blood stages. Severe malaria is an inflammatory cytokine driven disease. There is remarkable evidence that tumour necrosis factor (TNF) and interleukin-1 are important contributors to
the systemic disease caused by the infectious agent Pf.
It was not clear which of the compounds
present in Pg-FET or ellagitannins could
antagonize the host inflammatory response.Pg-FET and its constituents inhibited the Hz induced-MMP-9
secretion (Table 1 A and B). Punicalagin at 1 and 10 μM inhibited the release of MMP-9 by 38% and 79%; the MMP-9 mRNA levels decreased accordingly
(Table 1 C and D). EA at 1 and 10 μM inhibited the release of MMP-9 by 52 and 66%, respectively, and
the MMP-9 expression by 56 and 65% (Table 1 E and F). EA inhibited Hz-induced promoter activity by
38% and 50% at 1 and 10 μM, respectively, while the
inhibitory effect of punicalagin was only statistically significant at 10 μM (-65%). The in vitro beneficial effects of the fruit rind of Punica granatum L. for the treatment of malaria include, in addition to the anti-parasitic activity, the ability to limit the excessive inflammatory response in the host, thus possibly limiting the risk of progression to the more severe form of the disease, including the onset of cerebral malaria.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Keywords:
Ellagitannins ; Punica granatum L. ; malaria ; metalloprotease-9 ; NF-kB ; monocyte
Elenco autori:
E. Sangiovanni, M. Dell'Agli, G.V. Galli, M. Bulgari, N. Basilico, S. Romeo, D. Bhattacharya, D. Taramelli, E. Bosisio
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