Docosahexaenoic acid modulates in vitro the inflammation of celiac disease in intestinal epithelial cells via the inhibition of cPLA2
Articolo
Data di Pubblicazione:
2011
Citazione:
Docosahexaenoic acid modulates in vitro the inflammation of celiac disease in intestinal epithelial cells via the inhibition of cPLA2 / O. Vincentini, M.G. Quaranta, M. Viora, C. Agostoni, M. Silano. - In: CLINICAL NUTRITION. - ISSN 0261-5614. - 30:4(2011), pp. 541-546.
Abstract:
Background & aims: The cytosolic phospolypase A2 (cPLA2) - dependent release of arachidonic acid (AA) from the intra-epithelial lymphocytes plays a pivotal role in arming lymphocytes to cytolysis in the immune response of celiac disease. However, little is known about the role of enterocytes in releasing AA.
Docosahexaenoic acid (DHA) is a long chain polyunsaturated fatty acid that counteracts many of the proinflammatory effect of AA. The aims of the present work were to evaluate if: 1) intestinal epithelial cells have a role in the celiac inflammation, releasing AA, and 2) if DHA is able to modulate the celiac inflammation, down-regulating the release of AA.
Methods: A human intestinal epithelial cell line (Caco-2)was exposed to gliadin peptides (PT-gl) (500 mg/ml) and DHA (2 mg/ml), both alone and simultaneously up to 24 h.
Results: The exposure of those cells to PT-gl alone resulted in an increased AA release, cycloxygenase-2 expression, cPLA2 activity and prostaglandin E2 and interleukin-8 release in culture medium, whereas the simultaneous exposure of the cells to DHA and PT-gl prevented the above-mentioned increases.
Conclusions: These results suggest that intestinal epithelial cells sustain the celiac inflammation, releasing AA when stimulated with gliadin and that DHA inhibits the AA release by these cells.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Arachidonic acid; Celiac disease; DHA; Intestinal epithelial cells; Mucosal inflammation
Elenco autori:
O. Vincentini, M.G. Quaranta, M. Viora, C. Agostoni, M. Silano
Link alla scheda completa: