Data di Pubblicazione:
2006
Citazione:
Genetic dissection of shoot development in maize / J. Krstajic, P.S. Manzotti, A.P.A. Giulini, D. Durantini, S. Dolfini, N. Rascio, N. La Rocca, G.A. Gavazzi, G. Consonni - In: Proceedings of the 50. Italian Society of Agricultural Genetics Annual Congress[s.l] : Società Italiana di Genetica Agraria, 2006. - ISBN 88-900622-7-4. (( Intervento presentato al 50. convegno Convegno della Società Italiana di Genetica Agraria tenutosi a Ischia nel 2006.
Abstract:
The isolation of genes affecting plant shoot formation is an important prerequisite for
understanding the logic of plant development as well as for manipulating plant architecture. To this
aim, maize transpositional mutagenesis has been adopted in our laboratory and has led to the
isolation of different developmental mutants that are currently under study. Two of them,
shootmeristemless (sml) and fused leaves (fdl), are presently described. Their locations, on the long
arm of chromosome 10 and chromosome 7 respectively, have been established by traditional B-A
translocation mapping and subsequently by linkage analysis with visible, as well as molecular,
markers.
The sml gene is a recessive mutation affecting shoot apical meristem maintenance and lateral
organ formation. Its introgression in different genetic backgrounds has highlighted the epistatic
interaction between sml and the unlinked distorted growth (dgr) locus. Seeds homozygous for both
sml and dgr loci have a shootless phenotype whereas Dgr/-sml/sml seeds produce plants with
altered fillotaxis and abnormal leaf morphogenesis. Microscopy analysis of young dgr primordia
will be presented showing that the mutation does not interfere with epidermis formation although
cell shape is less regular than in the wild-type.
The fdl mutation confers a pleiotropic phenotype to the maize plant, which is specifically
confined to the juvenile phase. The lack of a regular coleoptile opening that in normal development
occurs after germination, is one of the fdl specific traits that we are presently investigating, for its
putative relationship with the programmed cell death (PCD) process. We will present preliminary
data on the cloning of the fdl gene along with a description of the strategy we have undertaken for
the isolation of novel alleles. They will constitute proof for the gene identity and will provide new
genetic material suitable for functional studies.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
shoot development ; maize ; shootmeristemless mutant ; fused leaves mutant
Elenco autori:
J. Krstajic, P.S. Manzotti, A.P.A. Giulini, D. Durantini, S. Dolfini, N. Rascio, N. La Rocca, G.A. Gavazzi, G. Consonni
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Titolo del libro:
Proceedings of the 50. Italian Society of Agricultural Genetics Annual Congress