Polymerase chain reaction products (PCR) on DNA barcode zone resolved by temporal temperature gradient electophoresis: a tool for species identification of mixed meat specimens : a technical note on preliminary results
Articolo
Data di Pubblicazione:
2011
Citazione:
Polymerase chain reaction products (PCR) on DNA barcode zone resolved by temporal temperature gradient electophoresis: a tool for species identification of mixed meat specimens : a technical note on preliminary results / F. Colombo, S. Chessa, P. Cattaneo, C.E.M. Bernardi. - In: FOOD CONTROL. - ISSN 0956-7135. - 22:8(2011), pp. 1471-1472.
Abstract:
The capability of PCR-TTGE to detect meat species in mixed animal samples was investigated as a necessary step in developing a method where the identification will be performed matching on the "DNA barcode" zone the sequences of resolved PCR products obtained from a limited set of "universal" primers. Exemplary mixtures from five important meat species were analyzed. At this stage more PCR reactions have to be applied on a sample but this should be easily improved using primers simultaneously (as a "cocktail") in a single reaction.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Meat species identification; Polymerase chain reaction; Temporal temperature gradient electrophoresis; DNA barcoding
Elenco autori:
F. Colombo, S. Chessa, P. Cattaneo, C.E.M. Bernardi
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