Isolation, proliferation, cytogenetic, and molecular characterization and In Vitro differentiation potency of canine stem cells from foetal adnexa : a comparative study of amniotic fluid, amnion, and umbilical cord matrix
Articolo
Data di Pubblicazione:
2011
Citazione:
Isolation, proliferation, cytogenetic, and molecular characterization and In Vitro differentiation potency of canine stem cells from foetal adnexa : a comparative study of
amniotic fluid, amnion, and umbilical cord matrix / M. Filioli Uranio, L. Valentini, A. Lange Consiglio, M. Caira, A.C. Guaricci, A. L’Abbate, C.R. Catacchio, M. Ventura, F. Cremonesi, M.E. Dell’Aquila. - In: MOLECULAR REPRODUCTION AND DEVELOPMENT. - ISSN 1040-452X. - 78:5(2011), pp. 361-373.
Abstract:
The possibility to isolate canine mesenchymal stem cells (MSCs) from foetal adnexa is interesting since several canine genetic disorders are reported to resemble similar dysfunctions in humans. In this study, we successfully isolated, cytogenetically and molecularly
characterized, and followed the differentiation potency of canine MSCs from foetal adnexa, such as amniotic fluid (AF), amniotic membrane (AM), and umbilical cord matrix (UCM). In the three types of cell lines, the morphology of proliferating cells typically appeared fibroblastlike,
and the population doubling time (DT) significantly increased with passage number. For AF- and AM-MSCs, cell viability did not change with passages. In UCM-MSCs, cell viability remained at approximately constant levels up to P6 and significantly decreased from P7(P<0.05). Amnion and UCM-MSCs expressed embryonic and MSC markers, such as Oct-4 CD44, CD184, and CD29, whereas AF-MSCs expressed Oct-4, CD44. Expression of the hematopoietic markers CD34 and CD45 was not found. Dog leucocyte antigens (DLADRA1
and DLA-79) were expressed only in AF-MSCs at P1. Isolated cells of the three cell
lines at P3 showed multipotent capacity, and differentiated in vitro into neurocyte, adipocyte, osteocyte,and chondrocyte, as demonstrated by specific stains and expression of molecular markers. Cells at P4 showed normal chromosomal number, structure, and telomerase activity. These results demonstrate that, in dog, MSCs can be successfully isolated from foetal adnexa and grown in vitro. Their proven stemness and chromosomal stability indicated
that MSCs could be used as a model to study stem cell biology and have an application in
therapeutic programs.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Canine mesenchymal stem cells ; foetal adnexa
Elenco autori:
M. Filioli Uranio, L. Valentini, A. Lange Consiglio, M. Caira, A.C. Guaricci, A. L’Abbate, C.R. Catacchio, M. Ventura, F. Cremonesi, M.E. Dell’Aquila
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