Morphometric characteristics and chromatin integrity of spermatozoa in three Italian dog breeds
Articolo
Data di Pubblicazione:
2010
Citazione:
Morphometric characteristics and chromatin integrity of spermatozoa in three Italian dog breeds / A. Lange-Consiglio, N. Antonucci, S. Manes, B. Corradetti, F. Cremonesi, D. Bizzaro. - In: JOURNAL OF SMALL ANIMAL PRACTICE. - ISSN 0022-4510. - 51:12(2010 Dec), pp. 624-627. [10.1111/j.1748-5827.2010.01001.x]
Abstract:
OBJECTIVES: Studies in many species indicate that variation of spermatozoan head morphology is a sensitive biomarker for abnormal chromatin structure and resultant clinical fertility. This preliminary
study evaluated spermatozoan head morphometry in different dog
breeds and assessed whether morphometric parameters could reflect spermatozoan DNA fragmentation in dogs.
METHODS: Spermatozoan morphometry and DNA quality (measured by TUNEL flow cytometry) were assessed in semen from 11 dogs of
three Italian breeds (Cirneco dell’Etna, Piccolo Levriero Italiano and
Segugio Maremmano).
RESULTS: Morphometric data showed that Segugio dogs had significantly larger (33·67%) spermatozoa and that Piccolo Levrieros had a higher incidence of long (46·75%) and elliptical spermatozoan
heads (11·5%) when compared with the samples from other breeds.
Moreover, the predominance of elliptical spermatozoa in one dog (23%) was significantly related to the percentage of spermatozoa with fragmented DNA (12·6%), whereas in another dog, where no more than 1% of spermatozoa was elliptical, only 0·36% of spermatozoa had damaged DNA. It is noteworthy that the breeding record
of the former dog in the previous 12 months showed poor fertility and fecundity.
CLINICAL SIGNIFICANCE: These data suggest that spermatozoan head morphometry could be breed related and that there is a significant
correlation between DNA fragmentation and elliptical spermatozoa in individual animals. This finding, albeit limited in our study to a single case, is possibly related to clinical infertility.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Spermatozoan head morphology ; biomarker ; DNA fragmentation ; Italian breed dogs
Elenco autori:
A. Lange-Consiglio, N. Antonucci, S. Manes, B. Corradetti, F. Cremonesi, D. Bizzaro
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