Ex vivo expansion of human circulating myogenic progenitors on cluster-assembled nanostructured TiO2
Articolo
Data di Pubblicazione:
2010
Citazione:
Ex vivo expansion of human circulating myogenic progenitors on cluster-assembled nanostructured TiO2 / M.L.C. Belicchi, S.A. Erratico, P. Razini, M.A. Meregalli, A. Cattaneo, E. Jacchetti, A. Farini, C. Villa, N. Bresolin, L. Porretti, C. Lenardi, P. Milani, Y. Torrente. - In: BIOMATERIALS. - ISSN 0142-9612. - 31:20(2010), pp. 5385-5396. [10.1016/j.biomaterials.2010.03.021]
Abstract:
Ex vivo expansion of hematopoietic stem cells has been explored in the fields of stem cell biology, gene therapy and clinical transplantation. Recently, we demonstrated the existence of a circulating myogenic progenitor expressing the CD133 antigen. The relative inability of circulating CD133+ stem cells to reproduce themselves ex vivo imposes substantial limitations on their use for clinical applications in muscular dystrophies. Here we report that the use of cluster-assembled nanostructured titanium dioxide (ns-TiO2) substrates, in combination with cytokine enriched medium, enables high-level expansion of circulating CD133+ stem cells in vitro. Furthermore, we demonstrate that expanded circulating CD133+ stem cells retain their in vitro capacity to differentiate into myogenic cells. The exploitation of cluster-assembled ns-TiO2 substrates for the expansion of CD133+ stem cells in vitro could therefore make the clinical application of these stem cells for the treatment of muscle diseases practical.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Stem cell; Titanium oxide; Nanotopography; Surface treatment; Cell culture; Muscular dystrophy
Elenco autori:
M.L.C. Belicchi, S.A. Erratico, P. Razini, M.A. Meregalli, A. Cattaneo, E. Jacchetti, A. Farini, C. Villa, N. Bresolin, L. Porretti, C. Lenardi, P. Milani, Y. Torrente
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