Data di Pubblicazione:
2010
Citazione:
Progesterone inhibits apoptosis in part by PGRMC1-regulated gene expression / J.J. Peluso, X. Liu, A. Gawkowska, V. Lodde, C.A. Wu. - In: MOLECULAR AND CELLULAR ENDOCRINOLOGY. - ISSN 0303-7207. - 320:1-2(2010 May 14), pp. 153-161.
Abstract:
Progesterone receptor membrane component-1 (PGRMC1) is present in both the cytoplasm and nucleus of spontaneously immortalized granulosa cells (SIGCs). PGRMC1 is detected as a monomer in the cytoplasm and a DTT-resistant PGRMC1 dimer in the nucleus. Transfected PGRMC1-GFP localizes mainly to the cytoplasm and does not form a DTT-resistant dimer. Moreover, forced expression of PGRMC1-GFP increases the sensitivity of the SIGCs to progesterone (P4)'s anti-apoptotic action, indicating that the PGRMC1 monomer is functional. However, when endogenous PGRMC1 is depleted by siRNA treatment and replaced with PGRMC1-GFP, P4 responsiveness is not enhanced, although overall levels of PGRMC1 are increased. P4's anti-apoptotic action is also attenuated by actinomycin D, an inhibitor of RNA synthesis, and P4 activation of PGRMC1 suppresses Bad and increases Bcl2a1d expression. Taken together, the present studies suggest a genomic component to PGRMC1's anti-apoptotic mechanism of action, which requires the presence of the PGRMC1 dimer.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Apoptosis; Granulosa cell; Membrane progesterone receptor; Progesterone
Elenco autori:
J.J. Peluso, X. Liu, A. Gawkowska, V. Lodde, C.A. Wu
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