Data di Pubblicazione:
2008
Citazione:
Enantioselective production of 3-hydroxy metabolites of tibolone by yeast reduction / D. Romano, V. Ferrario, D. Mora, R. Lenna, F. Molinari. - In: STEROIDS. - ISSN 0039-128X. - 73:1(2008), pp. 112-115. [10.1016/j.steroids.2007.09.008]
Abstract:
The enantioselective reduction of tibolone into the corresponding 3α-hydroxy or 3β-hydroxy metabolite can be controlled by choosing suited strains of yeasts and biotransformation conditions. A restricted screening performed among 52 yeasts showed that the 3α-epimer was preferentially obtained with high epimeric purity with various strains (i.e. with Kluyveromyces lactis CBS 2359), while only Saccharomyces cerevisiae CBS 3093 gave the 3β-epimer as major product. The reduction of tibolone with K. lactis CBS 2359 and S. cerevisiae CBS 3093 was optimised. S. cerevisiae CBS 3093 furnished a 96:4 ratio of 3β/3α with complete molar conversion within 72 h when the initial concentration of substrate was below 2.5 g/L. K. lactis CBS 2359 gave a 99:1 ratio of 3α/3β with complete conversion in 64 h.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Enantioselective reduction ; Saccharomyces cerevisiae ; Kluyveromyces lactis ; Tibolone ; 17alfa-Hydroxy-7alfa-methyl-19- ; norpregn-5(10)-en-20-in-3-one ; 15beta,7alfa-Methyl-19-norpregn-5(10)- ; en-20 in-3beta,17beta-diol ; 7alfa-Methyl-19-norpregn-5(10)-en-20- ; in-3beta,17beta-diol
Elenco autori:
D. Romano, V. Ferrario, D. Mora, R. Lenna, F. Molinari
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