Identification of suitable endogenous controls and differentially expressed microRNAs in canine fresh-frozen and FFPE lymphoma samples
Articolo
Data di Pubblicazione:
2010
Citazione:
Identification of suitable endogenous controls and differentially expressed microRNAs in canine fresh-frozen and FFPE lymphoma samples / M. Mortarino, G. Gioia, M.E. Gelain, F. Albonico, P. Roccabianca, E. Ferri, S. Comazzi. - In: LEUKEMIA RESEARCH. - ISSN 0145-2126. - 34:8(2010 Aug), pp. 1070-1077. [10.1016/j.leukres.2009.10.023]
Abstract:
The elucidation of microRNA (miRNA) expression pattern in canine lymphoma is
attractive for veterinary and comparative oncology due to similar genetics,
physiology and exposure to environment in dogs and humans. In this work, the
expression of a panel of mature miRNAs was quantitated in fresh-frozen and
formalin-fixed paraffin-embedded (FFPE) lymph nodes from canine lymphoma. The
major findings were: the detection of a panel of miRNAs expressed in canine lymph
node; the identification of three suitable endogenous controls (let-7a, miR-16,
and miR-26b) by NormFinder and geNorm analysis; the concordance between results
obtained from fresh-frozen and FFPE samples; the detection of upregulation of
miR-17-5p and miR-181a in B- and T-cell lymphomas respectively. This is the first
study aimed to the application of miRNAs analysis in canine lymphoma.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
MicroRNA; lymphoma; dog; reverse transcription quantitative PCR
Elenco autori:
M. Mortarino, G. Gioia, M.E. Gelain, F. Albonico, P. Roccabianca, E. Ferri, S. Comazzi
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