Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts
Articolo
Data di Pubblicazione:
2010
Citazione:
Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts / E. Fusi, R. Rebucci, C. Pecorini, A. Campagnoli, L. Pinotti, F. Saccone, F. Cheli, S. Purup, K. Sejrsen, A. Baldi. - In: TOXINS. - ISSN 2072-6651. - 2:6(2010 Jun 01), pp. 1265-1278. [10.3390/toxins2061265]
Abstract:
The aims of the current study were to determine the half-lethal concentration of ochratoxin A (OTA) as well as the levels of lactate dehydrogenase release and DNA fragmentation induced by OTA in primary porcine fibroblasts, and to examine the role of αtocopherol in counteracting its toxicity. Cells showed a dose-, time- and origin-dependent (ear vs. embryo) sensitivity to ochratoxin A. Pre-incubation for 3 h with 1 nM αtocopherol significantly (P < 0.01) reduced OTA cytotoxicity, lactate dehydrogenase release and DNA damage in both fibroblast cultures. These findings indicate that α-tocopherol supplementation may counteract short-term OTA toxicity, supporting its defensive role in the cell membrane.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
E. Fusi, R. Rebucci, C. Pecorini, A. Campagnoli, L. Pinotti, F. Saccone, F. Cheli, S. Purup, K. Sejrsen, A. Baldi
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