Engraftment of Bone Marrow-Derived Stem Cells to the Lung in a Model of Acute Respiratory Infection by Pseudomonas Aeruginosa
Articolo
Data di Pubblicazione:
2009
Citazione:
Engraftment of Bone Marrow-Derived Stem Cells to the Lung in a Model of Acute Respiratory Infection by Pseudomonas Aeruginosa / J. Rejman, C. Colombo, M. Conese. - In: MOLECULAR THERAPY. - ISSN 1525-0016. - 17:7(2009 Jul), pp. 1257-1265. [10.1038/mt.2009.96]
Abstract:
Stem cell therapy presents an attractive approach to cure cystic fibrosis (CF) lung disease. We set out to investigate the effect of epithelial damage caused by Pseudomonas aeruginosa, a pathogenic bacterium widely occurring in CF, on the engraftment of bone marrow cells in airway epithelium. Intravenous or intratracheal administration of unfractionated green fluorescent protein (GFP+) bone marrow cells in P. aeruginosa-infected mice resulted in none or very few GFP+ cells detected in the lungs of the recipient mice, respectively. Only when GFP+ bone marrow cells were purified to obtain a cell suspension enriched in progenitor cells and injected intratracheally, significant numbers of GFP+ cells were detected. Localization of the donor cells at the level of airway epithelium was confirmed by Y-chromosome fluorescence in situ hybridization (FISH) analysis. All donor-derived Y-chromosome+ cells were found to express cytokeratin (CK). The fractions of GFP+ cells expressing CK were 0.34 and 0.76% for the 105 and 106 colony forming units (cfu) bacterial inoculums, respectively. When scored by Y-chromosome positivity these numbers were 0.60 and 1.12%, respectively. Our results show for the first time that tissue damage inflicted by bacteria like P. aeruginosa facilitates the airway engraftment of heterologous bone marrow-derived stem cells and their epithelial transformation.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
J. Rejman, C. Colombo, M. Conese
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