Protein tyrosine nitration is triggered by nerve growth factor during neuronal differentiation of PC12 cells
Articolo
Data di Pubblicazione:
2003
Citazione:
Protein tyrosine nitration is triggered by nerve growth factor during neuronal differentiation of PC12 cells / G. Cappelletti, M.G. Maggioni, G. Tedeschi, R. Maci. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - 288:1(2003), pp. 9-20. [10.1016/S0014-4827(03)00209-X]
Abstract:
Nitric oxide (NO) is a signaling molecule implicated in a spectrum of cellular processes including neuronal
differentiation. The signaling pathway triggered by NO in physiological processes involves the activation of
soluble guanylate cyclase and S-nitrosylation of proteins, and, as recently proposed, nitration of tyrosine
residues in proteins. However, little is known about the mechanisms involved and the target proteins for
endogenous NO during the progression of neuronal differentiation. To address this question, we investigated
the presence, localization, and subcellular distribution of nitrated proteins during neurotrophin-induced
differentiation of PC12 cells. We find that some proteins show basal levels of tyrosine nitration in PC12 cells
grown in the absence of nerve growth factor (NGF) and that nitration levels increase significantly after 2 days
of incubation with this neurotrophin. Nitrated proteins accumulate over a period of several days in the
presence of NGF. We demonstrate that this nitration is coupled to activation of nitric oxide synthase. The
subcellular distribution of nitrated proteins changes during PC12 cell differentiation, displaying a shift from the
cytosolic to the cytoskeletal fraction and we identified a-tubulin as the major target of nitration in PC12 cells by
N-terminal sequence and MALDI-TOF analyses. We conclude that tyrosine nitration of proteins could be a
novel molecular mechanism involved in the signaling pathway by which NO modulates NGF-induced
differentiation in PC12 cells.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
α-Tubulin; Neuronal differentiation; Neurotrophin; Nitric oxide; Nitrotyrosine
Elenco autori:
G. Cappelletti, M.G. Maggioni, G. Tedeschi, R. Maci
Link alla scheda completa: