Data di Pubblicazione:
2008
Citazione:
Identification of new p63 targets in keratinocytes by ChIp on chip / M.A. Viganò, F. Zambelli, R. Mantovani. ((Intervento presentato al 4. convegno SIBBM Seminar "Frontiers in Molecular Biology" tenutosi a Milano nel 2008.
Abstract:
p63 is a transcription factor required for the development and maintenance of
ectodermal tissues, including skin, limb and, in general, multilayered
epithelia. The identification of its target genes is fundamental for
understanding the complex network of gene regulation governing the
development of these epithelia. Our lab employed two strategies for this task:
expression profiling of si RNA p63 depleted human keratinocytes (HaCat cells)
(1) and ChIP on chIP analysis on two different platforms (CpG island and
promoter arrays) with specific p63 antibodies in HaCat cells. We previously
reported 186 high confidence p63 targets which were validated in different
biological assays.(2). A re-analysis of the chip-chip data with less stringent
criteria enlarged this list of putative targets to almost 1000 new locations.
Independent chIP validations confirmed that around 60-70% of these new
targets are bound by p63 in vivo and a subset of these genes are changing in
expression during in vitro differentiation of primary human keratinocytes.
Moreover, functional annotation of this extended list highlighted some
unexpected GO terms enrichments such as heart development and nucleo-
cytoplasmic transport. Detailed location analysis and functional studies are on
going on these particular subclasses of genes to understand the physiological
role of p63 in these processes.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
M.A. Viganò, F. Zambelli, R. Mantovani
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