Regulation of Saccharomyces Rad53 checkpoint kinase during adaptation from DNA damage-induced G2/M arrest
Articolo
Data di Pubblicazione:
2001
Citazione:
Regulation of Saccharomyces Rad53 checkpoint kinase during adaptation from DNA damage-induced G2/M arrest / A. Pellicioli, S.E. Lee, C. Lucca, M. Foiani, J.E. Haber. - In: MOLECULAR CELL. - ISSN 1097-2765. - 7:2(2001), pp. 293-300. [10.1016/S1097-2765(01)00177-0]
Abstract:
Saccharomyces cells with one unrepaired double-strand break (DSB) adapt after checkpoint-mediated G2/M arrest. Adaptation is accompanied by loss of Rad53p checkpoint kinase activity and Chk1p phosphorylation. Rad53p kinase remains elevated in yku70Δ and cdc5-ad cells that fail to adapt. Permanent G2/M arrest in cells with increased single-stranded DNA is suppressed by the rfa1-t11 mutation, but this RPA mutation does not suppress permanent arrest in cdc5-ad cells. Checkpoint kinase activation and inactivation can be followed in G2-arrested cells, but there is no kinase activation in G1-arrested cells. We conclude that activation of the checkpoint kinases in response to a single DNA break is cell cycle regulated and that adaptation is an active process by which these kinases are inactivated.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
A. Pellicioli, S.E. Lee, C. Lucca, M. Foiani, J.E. Haber
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