Data di Pubblicazione:
2004
Citazione:
Cell-cycle regulation of NF-YC nuclear localization / M. Frontini, C. Imbriano, I. Manni, R. Mantovani. - In: CELL CYCLE. - ISSN 1538-4101. - 3:2(2004), pp. 217-222.
Abstract:
NF-Y is a trimeric activator with histone fold-HFM-subunits that binds to the CCAAT-box and is required for a majority of cell cycle promoters, often in conjuction with E2Fs. In vivo binding of NF-Y is dynamic during the cell cycle and correlates with gene activation. We performed immunofluorescence studies on endogenous, GFP- and Flag-tagged overexpressed NF-Y subunits. NF-YA, NF-YB are nuclear proteins. Unexpectedly, NF-YC localizes both in cytoplamatic and nuclear compartments and its nuclear localization is determined by the interaction with its heterodimerization partner NF-YB. Most importantly, compartmentalization is regulated during the cell cycle of serum restimulated NIH3T3 cells, accumulating in the nucleus at the onset of S phase. These data point to the control of HFM heterodimerization as an important layer of NF-Y regulation during cell cycle progression.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
CCAAT; Histone fold; NF-Y
Elenco autori:
M. Frontini, C. Imbriano, I. Manni, R. Mantovani
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