Potential advantages of cell administration on the inflammatory response compared to standard ACE inhibitor treatment in experimental myocardial infarction
Articolo
Data di Pubblicazione:
2008
Citazione:
Potential advantages of cell administration on the inflammatory response compared to standard ACE inhibitor treatment in experimental myocardial infarction / M.M. Ciulla, E. Montelatici, S. Ferrero, P. Braidotti, R. Paliotti, G. Annoni, E. De Camilli, G. Busca, L. Chiappa, P. Rebulla, F. Magrini, L. Lazzari. - In: JOURNAL OF TRANSLATIONAL MEDICINE. - ISSN 1479-5876. - 6(2008 Jun 12). [10.1186/1479-5876-6-30]
Abstract:
BACKGROUND: Bone Marrow (BM) progenitor cells can target the site of myocardial injury, contributing to tissue repair by neovascolarization and/or by a possible direct paracrine effect on the inflammatory cascade. Angiotensin Converting Enzyme inhibitors (ACE-I) are effective in reducing mortality and preventing left ventricular (LV) function deterioration after myocardial infarction. METHODS: We investigated the short term effects of BM mononuclear cells (BMMNCs) therapy on the pro-inflammatory cytokines (pro-CKs) and on LV remodelling and compared these effects over a standard ACE-I therapy in a rat model of myocardial cryodamage.Forty two adult inbread Fisher-F344 rats were randomized into three groups: untreated (UT; n = 12), pharmacological therapy (ACE-I; n = 14, receiving quinapril), and cellular therapy (BMMNCs; n = 16, receiving BMMNCs infusion). Rats underwent to a standard echocardiogram in the acute setting and 14 days after the damage, before the sacrifice. Pro-CKs analysis (interleukin (IL)1beta, IL-6, tumor necrosis factor (TNF)alpha was performed (multiplex proteome arrays) on blood samples obtained by direct aorta puncture before the sacrifice; a control group of 6 rats was considered as reference. RESULTS: Concerning the extension of the infarcted area as well as the LV dimensions, no differences were observed among the animal groups; treated rats had lower left atrial diameters and higher indexes of LV function. Pro-Cks were increased in infarcted-UT rats if compared with controls, and significantly reduced by BMMNCs and ACE-I ; TNFalpha inversely correlated with LV fractional shortening. CONCLUSION: After myocardial infarction, both BMMNCs and ACE-I reduce the pattern of pro-Ck response, probably contributing to prevent the deterioration of LV function observed in UT rats.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
M.M. Ciulla, E. Montelatici, S. Ferrero, P. Braidotti, R. Paliotti, G. Annoni, E. De Camilli, G. Busca, L. Chiappa, P. Rebulla, F. Magrini, L. Lazzari
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